Kolloquiumsvortrag, Dr. Seraphine Wegner, MPI Polymer-Forschung Mainz / am 09.01.2017

09.01.2016 von 17:15 bis 18:45

Technische Fakultät, Institut für Materialwissenschaft, Kaiserstr. 2, Kiel, Raum: "Aquarium", Geb. D

Titel: Photoswitchable linkers for cell and protein patterning

Abstract: Cell adhesion is regulated both in space and time during many biological processes such as embryogenesis, wound healing, cancer invasion and tissue formation. Therefore, it is of great importance to develop platforms to reversibly control cell interactions in vitro and in vivo non-invasively and with high precision. Light is the ideal switch towards this goal as it is in general considered biorthogonal and allows for very  high spatial and temporal control.  Here, we report a number of platforms that allow controlling cell-surface interactions with light to guide cell adhesion and behavior based on photocleavable nitrobenzene analogues and light responsive proteins. We attach these light responsive molecules specifically to PEG coated surfaces and are able to cleave off the presented functionality or attach it to the surface upon illumination with light. In micropatterning cRGD on these surfaces, we can control the cell adhesions and prevent cell attachment to specific regions on the substrate. Similarly, we can controllably present proteins of interest on such surfaces with any micropattern. These newly developed light responsive platforms, offer great flexibility to microstructure interphases with different functional molecules and control cell-surface interactions with great precision.

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